Functional Conversion of Hemocyanin to Phenoloxidase by Horseshoe Crab Antimicrobial Peptides
نویسندگان
چکیده
منابع مشابه
Functional conversion of hemocyanin to phenoloxidase by horseshoe crab antimicrobial peptides.
Arthropod hemocyanins and phenoloxidases serve different physiological functions as oxygen transporters and enzymes involved in defense reactions, respectively. However, they are equipped with a structurally similar oxygen-binding center. We have shown that the clotting enzyme of the horseshoe crab, Tachypleus tridentatus, functionally converts hemocyanin to phenoloxidase by forming a complex w...
متن کاملHemocyanin of the Horseshoe Crab, Limzdus polyphemus
The high molecular weight hemocyanin found in the hemolymph of the horseshoe crab, Limulus polyphemus, is composed of at least eight different kinds of subunits. Ion exchange chromatography at high pH in the presence of EDTA yields five major zones, hemocyanins I to V, three of which are electrophoretically heterogeneous. The subunits have similar molecular weights, 65,000 to 70,000, and their ...
متن کاملTarantula hemocyanin shows phenoloxidase activity.
An enzyme generally catalyzes one well defined reaction with high specificity and efficiency. We report here in contrast that the copper protein hemocyanin of the tarantula Eurypelma californicum exhibits two different functions. These occur at the same active site. While hemocyanin usually is an oxygen carrier, its function can be transformed totally to monophenoloxidase and o-diphenoloxidase ...
متن کاملProtecting Horseshoe Crab Habitat
Each spring adult horseshoe crabs migrate from deep bay waters and the Atlantic continental shelf to intertidal sandy beaches to spawn. Horseshoe crabs are an important food source for migrating shorebirds that feed on the eggs, for migrating fish that feed on the eggs and larvae and for Atlantic loggerhead turtles that feed on horseshoe crabs while using the Chesapeake Bay as a summer nursery ...
متن کاملSDS-induced phenoloxidase activity of Helix aspersa maxima hemocyanin
Oxygen-transporting protein of the hemolimph of snails Helix aspersa maxima (HaH) was converted from being an oxygen carrier to a form which exhibited phenoloxidase activity by incubation with sodium dodecyl sulfate (SDS). On treatment with 1.73 mM SDS, for 3 min, significant increase of catalytic efficiency of hemocyanin towards substrate catechol (kcat/Km = 34.56 mM.min -1 versus kcat/Km = 0....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m102596200